ok, so you have changed the 2 port valve for heating.
do you have 2, 2 port valves?
did you replace the actuator on the 2 port valve, as some honeywell valves come as 2 separate parts (actuator & valve body)
when you turn the heating on does the actuator move?
my first instict would be that the microswitch in the actuator isn't making, even if it is new.
if you get heat from the radiators with hot water on then that pretty much tells us that the valve is opening, so therfore i'd think it's pretty safe to assume the timer and room stat are sending power to open the valve, it's just that the power isn't getting to the boiler, which point's to the microswitch.
however if you've been in about the wiring centre, it may be possible that you've got some wires in the wrong place, it's easily done.
first thing to check is that the orange wire coming from the actuator is going into the terminal for thelive to the boiler & the pump (usually brown).
if you have 2 2 port valves then both orange wires should be in the same terminal in the wiring centre.
hope that helps.
Ian, February 2010