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When should I re-seal my windows?

I have wood windo surrounds and Double glazing. As the seals were mouldy and want to paint the surrounds, I have stripped all the old mouldy seal and varnish. When should I re-seal? Before the primer/undercoat or inbetween coates of paints or at the end? Which is the easist seal to use and the most efficient and long lasting?
Ursula, September 2009